Increased riboflavin production from activated bleaching earth by a mutant strain of Ashbya gossypii
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منابع مشابه
Increased riboflavin production from activated bleaching earth by a mutant strain of Ashbya gossypii.
The production of riboflavin from vegetable oil was increased using a mutant strain of Ashbya gossypii. This mutant was generated by treating the wild-type strain with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Riboflavin production was 10-fold higher in the mutant compared to the wild-type strain. The specific intracellular catalase activity after 3 d of culture was 6-fold higher in the muta...
متن کاملIsolation and characterization of an Ashbya gossypii mutant for improved riboflavin production
The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to ribo...
متن کاملComparative metabolic flux analysis of an Ashbya gossypii wild type strain and a high riboflavin-producing mutant strain.
In the present study, we analyzed the central metabolic pathway of an Ashbya gossypii wild type strain and a riboflavin over-producing mutant strain developed in a previous study in order to characterize the riboflavin over-production pathway. (13)C-Metabolic flux analysis ((13)C-MFA) was carried out in both strains, and the resulting data were fit to a steady-state flux isotopomer model using ...
متن کاملGenome scale metabolic modeling of the riboflavin overproducer Ashbya gossypii.
Ashbya gossypii is a filamentous fungus that naturally overproduces riboflavin, or vitamin B2. Advances in genetic and metabolic engineering of A. gossypii have permitted the switch from industrial chemical synthesis to the current biotechnological production of this vitamin. Additionally, A. gossypii is a model organism with one of the smallest eukaryote genomes being phylogenetically close to...
متن کاملImprovement of Riboflavin Production Using Mineral Support in the Culture of Ashbya gossypii
Riboflavin production in a culture of Ashbya gossypii was enhanced by adding mineral support with adsorbed soybean oil. When the support in an amount of 1 % to the amount of medium was added into the culture at agitation intensity corresponding to impeller rotation rate of 600 rpm, the attained riboflavin concentration was 2.5 g/L at the culture time of 4 days, i.e. 1.6 times higher than that i...
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ژورنال
عنوان ژورنال: Journal of Bioscience and Bioengineering
سال: 2009
ISSN: 1389-1723
DOI: 10.1016/j.jbiosc.2009.04.021